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Hans-Peter Kiem, MD, Dr.Med.

NGEC Principal Investigator

Member, Clinical Research Division, Fred Hutchinson Cancer Research Center
Investigator, Markey Molecular Medicine Center, University of Washington
Professor, University of Washington School of Medicine
Adjunct Professor of Pathology, University of Washington School of Medicine

 
 

 
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N., D1-100
PO Box 19024
Seattle, WA 98109-1024
Tel: 206-667-4425; Fax: 206-667-6124

E-mail Dr. Kiem

Dr. Kiem is a full member at the Fred Hutchinson Cancer Research Center; an investigator at the Markey Molecular Medicine Center; Professor of Medicine at the University of Washington; and Adjunct Professor of Pathology at the University of Washington. His laboratory studies stem cell biology and stem cell gene transfer with the goal of developing novel stem cell–based treatment strategies for patients with genetic, infectious and malignant diseases. Current research in his laboratory focuses on stem cell biology/transplantation and gene therapy studies to: 1) characterize HSCs from large animal models; 2) develop preclinical protocols using stem cell gene therapy to facilitate allogeneic nonmyeloablative stem cell transplantation; and 3) develop clinical gene therapy protocols for genetic and acquired diseases including cancer.

Dr. Kiem is the principal investigator for the NGEC in the area of gene repair in canine immunodeficiency models.
 

"The NGEC provides a unique opportunity to study homing endonuclease-based hematopoietic stem cell gene-repair strategies in a clinically relevant animal model."
Hans-Peter Kiem

Awards and Honors

  • Fellowship from the Deutsche Krebshilfe (German Cancer Aid): 1988
  • Markey Charitable Trust Award: 1995
  • Editorial Board Memberships: Blood (2002–present); Molecular Therapy (2003–present); Gene Therapy (2003–present)
  • Member, Immunology and Hematopoietic Cell Gene Therapy Committee, American Society of Gene Therapy: 2003-2006
  • Chair, Gene Therapy Subcommittee, International Society for Cellular Therapy: 2004
  • Member, Developmental Therapeutics Study Section: 2004–present
  • Member, American Society of Clinical Investigation: 2005–present

Areas of Expertise

  • Stem cell biology/transplantation
  • Gene therapy
  • Vector development

Research Interests

  • Characterize HSCs from large animal models
  • Analyze the clonal composition of hematopoiesis after transplantation of gene-modified HSCs
  • Develop novel vector systems for improved gene delivery
  • Study retroviral integration sites and insertional mutaganesis
  • Stem cell expansion and cord blood transplantation
  • Nonhuman primate embryonic stem cells
  • Develop pre-clinical protocols using stem cell gene therapy to facilitate allogeneic nonmyeloablative stem cell transplantation
  • Develop clinical gene therapy protocols for brain tumors, Fanconi anemia and AIDS

Overview of the Kiem Lab

The main focus of the Kiem Laboratory is to study stem cell biology and stem cell gene transfer with the goal of developing novel stem cell–based treatment strategies for patients with genetic, infectious and malignant diseases.

 

NGEC Research

Dr. Kiem and his team will initiate studies of LHE-based gene modification in canine hematopoietic stem cells as an initial step towards translation of gene repair of hematopoietic cells to humans. These studies will involve the following specific aims:

  1. The Kiem lab will evaluate initial and stable genomic marking at endogenous and artificial reporter loci in canine hematopoietic stem cells using NIL lentiviral vectors expressing available LHEs.
  2. Using marking conditions established in Aim #1, the Kiem Lab will evaluate non-myeloablative conditioning regimens and a “knock-in” approach with an in vivo selectable marker system.
  3. Using the methods developed in the above aims, the Kiem Lab will pilot gene repair of the naturally-arising canine lymphohematopoietic diseases XSCID and PK deficiency. These studies will involve generation via the NGEC Cell and Virus CORE of appropriate NIL vectors expressing LHEs (designed in Aim #1) which cut at the XSCID and PK loci, followed by use of these vectors for stem cell transduction, and evaluation of the efficacy of correction of the canine XSCID and PK disease phenotypes.

Key personnel carrying out this research include Nina Munoz (postdoctoral fellow) and Brian Beard (associate in clinical research).

Publications

2013

Peterson CW, Younan P, Jerome KR, Kiem H-P.  2013.  Combinatorial anti-HIV gene therapy: using a multipronged approach to reach beyond HAART.. Gene Ther.
Berger C, Berger M, Beard BC, Kiem H-P, Gooley TA, Riddell SR.  2013.  Proliferation-Linked Apoptosis of Adoptively Transferred T Cells after IL-15 Administration in Macaques.. PLoS One. 8(2):e56268.
Stone D, Kiem H-P, Jerome KR.  2013.  Targeted gene disruption to cure HIV.. Curr Opin HIV AIDS.
Watts KL, Delaney C, Nelson V, Trobridge GD, Beard BC, Humphries KR, Kiem H-P.  2013.  CD34(+) Expansion With Delta-1 and HOXB4 Promotes Rapid Engraftment and Transfusion Independence in a Macaca nemestrina Cord Blood Transplant Model.. Mol Ther.

2012

Trobridge GD, Horn PA, Beard BC, Kiem H-P.  2012.  Large animal models for foamy virus vector gene therapy.. Viruses. 4(12):3572-88.
Muñoz NM, Beard BC, Ryu BY, Luche RM, Trobridge GD, Rawlings DJ, Scharenberg AM, Kiem H-P.  2012.  Novel reporter systems for facile evaluation of I-SceI-mediated genome editing.. Nucleic acids research. 40(2):e14.
Tolar J, Becker PS, Bueren J, Clapp WD, Hanenberg H, de Heredia CD, Kiem H-P, Navarro S, Qasba P, Río P et al..  2012.  Gene Therapy for Fanconi Anemia: One Step Closer to the Clinic.. Human gene therapy.
Mrugala MM, Adair JE, Kiem H-P.  2012.  Outside the box-novel therapeutic strategies for glioblastoma.. Cancer journal (Sudbury, Mass.). 18(1):51-8.
Kiem H-P, Jerome KR, Deeks SG, McCune JM.  2012.  Hematopoietic-Stem-Cell-Based Gene Therapy for HIV Disease.. Cell stem cell. 10(2):137-47.
Adair JE, Beard BC, Trobridge GD, Neff T, Rockhill JK, Silbergeld DL, Mrugala MM, Kiem H-P.  2012.  Extended Survival of Glioblastoma Patients After Chemoprotective HSC Gene Therapy.. Science translational medicine. 4(133):133ra57.
Gori JL, Beard BC, Ironside C, Karponi G, Kiem H-P.  2012.  In vivo selection of autologous MGMT gene-modified cells following reduced-intensity conditioning with BCNU and temozolomide in the dog model.. Cancer gene therapy.
Corrigan-Curay J, Cohen-Haguenauer O, O'Reilly M, Ross SR, Fan H, Rosenberg N, Somia N, King N, Friedmann T, Dunbar C et al..  2012.  Challenges in vector and trial design using retroviral vectors for long-term gene correction in hematopoietic stem cell gene therapy.. Molecular therapy : the journal of the American Society of Gene Therapy. 20(6):1084-94.
Adair JE, Zhao X, Chien S, Fang M, Wohlfahrt ME, Trobridge GD, Taylor JA, Beard BC, Kiem H-P, Becker PS.  2012.  Cyclophosphamide promotes engraftment of gene-modified cells in a mouse model of Fanconi anemia without causing cytogenetic abnormalities.. Journal of molecular medicine (Berlin, Germany).
Luche RM, Enssle J, Kiem H-P.  2012.  Abrogated cryptic activation of lentiviral transfer vectors.. Scientific reports. 2:438.
Digiusto DL, Kiem H-P.  2012.  Current translational and clinical practices in hematopoietic cell and gene therapy.. Cytotherapy. 14(7):775-90.
Thakar MS, Bonfim C, Sandmaier BM, O'Donnell P, Ribeiro L, Gooley T, Deeg HJ, Flowers ME, Pasquini R, Storb R et al..  2012.  Cyclophosphamide-Based In Vivo T-Cell Depletion for HLA-Haploidentical Transplantation in Fanconi Anemia.. Pediatr Hematol Oncol.
Gori JL, Chandrasekaran D, Kowalski JP, Adair JE, Beard BC, D'Souza SL, Kiem H-P.  2012.  Efficient generation, purification, and expansion of CD34+ hematopoietic progenitor cells from nonhuman primate induced pluripotent stem cells.. Blood.
Certo MT, Gwiazda KS, Kuhar R, Sather B, Curinga G, Mandt T, Brault M, Lambert AR, Baxter SK, Jacoby K et al..  2012.  Coupling endonucleases with DNA end-processing enzymes to drive gene disruption.. Nat Methods.
Trobridge GD, Beard BC, Wu RA, Ironside C, Malik P, Kiem H-P.  2012.  Stem Cell Selection In Vivo Using Foamy Vectors Cures Canine Pyruvate Kinase Deficiency.. PLoS One. 7(9):e45173.

2011

Okazuka K, Beard BC, Emery DW, Schwarzwaelder K, Spector MR, Sale GE, von Kalle C, Kiem H-P, Blau AC.  2011.  Long-term Regulation of Genetically Modified Primary Hematopoietic Cells in Dogs.. Molecular therapy : the journal of the American Society of Gene Therapy.
Okazuka K, Beard BC, Emery DW, Schwarzwaelder K, Spector MR, Sale GE, von Kalle C, Torok-Storb B, Kiem H-P, Blau AC.  2011.  Corrigendum to "Long-term Regulation of Genetically Modified Primary Hematopoietic Cells in Dogs".. Molecular therapy : the journal of the American Society of Gene Therapy. 19(11):2102.
Zhong B, Trobridge GD, Zhang X, Watts KL, Ramakrishnan A, Wohlfahrt M, Adair JE, Kiem H-P.  2011.  Efficient Generation of Nonhuman Primate Induced Pluripotent Stem Cells.. Stem cells and development.
Imren S, Zhang X-B, Humphries KR, Kiem H-P.  2011.  Insights into leukemia-initiating cell frequency and self-renewal from a novel canine model of leukemia.. Experimental hematology. 39(1):124-32.
Watts KL, Nelson V, Wood BL, Trobridge GD, Beard BC, Humphries KR, Kiem H-P.  2011.  Hematopoietic stem cell expansion facilitates multilineage engraftment in a nonhuman primate cord blood transplantation model.. Experimental hematology.
Inamoto Y, Flowers MED, Appelbaum FR, Carpenter PA, Deeg JH, Furlong T, Kiem H-P, Mielcarek M, Nash RA, Storb RF et al..  2011.  A Retrospective Comparison of Tacrolimus versus Cyclosporine with Methotrexate for Immunosuppression after Allogeneic Hematopoietic Cell Transplantation with Mobilized Blood Cells.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation.
Tolar J, Adair JE, Antoniou M, Bartholomae CC, Becker PS, Blazar BR, Bueren J, Carroll T, Cavazzana-Calvo M, Clapp WD et al..  2011.  Stem Cell Gene Therapy for Fanconi Anemia: Report from the 1st International Fanconi Anemia Gene Therapy Working Group Meeting.. Molecular therapy : the journal of the American Society of Gene Therapy.
Zhong B, Watts KL, Gori JL, Wohlfahrt ME, Enssle J, Adair JE, Kiem H-P.  2011.  Safeguarding Nonhuman Primate iPS Cells With Suicide Genes.. Molecular therapy : the journal of the American Society of Gene Therapy.
Persson J, Beyer I, Yumul R, Li ZY, Kiem H-P, Roffler S, Lieber A.  2011.  Immuno-Therapy with Anti-CTLA4 Antibodies in Tolerized and Non-Tolerized Mouse Tumor Models.. PloS one. 6(7):e22303.
Watts K, Zhang X, Beard B, Chiu SY, Trobridge GD, Humphries KR, Kiem H-P.  2011.  Differential Effects of HOXB4 and NUP98-HOXA10hd on Hematopoietic Repopulating Cells in a Nonhuman Primate Model.. Human gene therapy.
Watts KL, Adair J, Kiem H-P.  2011.  Hematopoietic stem cell expansion and gene therapy.. Cytotherapy. 13(10):1164-71.

2010

Mrugala MM, Adair J, Kiem H-P.  2010.  Temozolomide: Expanding its role in brain cancer.. Drugs of today (Barcelona, Spain : 1998). 46(11):833-46.
Thakar MS, Santos EB, Gooley TA, Sale G, Storb R, Kiem H-P, Sandmaier BM.  2010.  Evaluation of posttransplant methotrexate to facilitate engraftment in the canine major histocompatibility complex-haploidentical nonmyeloablative transplant model.. Transplantation. 90(1):14-22.
Sangiolo D, Storb R, Deeg JH, Flowers MED, Martin PJ, Sandmaier BM, Kiem H-P, Nash RA, Doney K, Leisenring WM et al..  2010.  Outcome of allogeneic hematopoietic cell transplantation from HLA-identical siblings for severe aplastic anemia in patients over 40 years of age.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 16(10):1411-8.
Watts KL, Delaney C, Humphries KR, Bernstein ID, Kiem H-P.  2010.  Combination of HOXB4 and Delta-1 ligand improves expansion of cord blood cells.. Blood. 116(26):5859-66.
Murnane R, Zhang XB, Hukkanen RR, Vogel K, Kelley S, Kiem HP.  2010.  Myelodysplasia in 2 Pig-Tailed Macaques (Macaca nemestrina) Associated With Retroviral Vector-Mediated Insertional Mutagenesis and Overexpression of HOXB4.. Veterinary pathology.
Thakar MS, Kurre P, Storb R, Kletzel M, Frangoul H, Pulsipher MA, Leisenring W, Flowers MED, Sandmaier BM, Woolfrey A et al..  2010.  Treatment of Fanconi anemia patients using fludarabine and low-dose TBI, followed by unrelated donor hematopoietic cell transplantation.. Bone marrow transplantation.
Beard BC, Trobridge GD, Ironside C, McCune JS, Adair JE, Kiem H-P.  2010.  Efficient and stable MGMT-mediated selection of long-term repopulating stem cells in nonhuman primates.. The Journal of clinical investigation. 120(7):2345-54.
Becker PS, Taylor JA, Trobridge GD, Zhao X, Beard BC, Chien S, Adair J, Kohn DB, Wagner JE, Shimamura A et al..  2010.  Preclinical correction of human Fanconi anemia complementation group A bone marrow cells using a safety-modified lentiviral vector.. Gene therapy. 17(10):1244-52.
Kiem H-P, Ironside C, Beard BC, Trobridge GD.  2010.  A retroviral vector common integration site between leupaxin and zinc finger protein 91 (ZFP91) observed in baboon hematopoietic repopulating cells.. Experimental hematology. 38(9):819-22,822.e1-3.
Trobridge GD, Kiem H-P.  2010.  Large animal models of hematopoietic stem cell gene therapy.. Gene therapy. 17(8):939-48.
Trobridge GD, Wu RA, Hansen M, Ironside C, Watts KL, Olsen P, Beard BC, Kiem H-P.  2010.  Cocal-pseudotyped lentiviral vectors resist inactivation by human serum and efficiently transduce primate hematopoietic repopulating cells.. Molecular therapy : the journal of the American Society of Gene Therapy. 18(4):725-33.
Enssle J, Trobridge GD, Keyser KA, Ironside C, Beard BC, Kiem H-P.  2010.  Stable marking and transgene expression without progression to monoclonality in canine long-term hematopoietic repopulating cells transduced with lentiviral vectors.. Human gene therapy. 21(4):397-403.
Kiem H-P, Wu RA, Sun G, von Laer D, Rossi JJ, Trobridge GD.  2010.  Foamy combinatorial anti-HIV vectors with MGMTP140K potently inhibit HIV-1 and SHIV replication and mediate selection in vivo.. Gene therapy. 17(1):37-49.

2009

Beard BC, Sud R, Keyser KA, Ironside C, Neff T, Gerull S, Trobridge GD, Kiem H-P.  2009.  Long-term polyclonal and multilineage engraftment of methylguanine methyltransferase P140K gene-modified dog hematopoietic cells in primary and secondary recipients.. Blood. 113(21):5094-103.
Trobridge GD, Allen J, Peterson L, Ironside C, Russell DW, Kiem H-P.  2009.  Foamy and lentiviral vectors transduce canine long-term repopulating cells at similar efficiency.. Human gene therapy. 20(5):519-23.
Beard BC, Kiem H-P.  2009.  Canine models of gene-modified hematopoiesis.. Methods in molecular biology (Clifton, N.J.). 506:341-61.
Hukkanen RR, Gillen M, Grant R, Liggitt DH, Kiem H-P, Kelley ST.  2009.  Simian varicella virus in pigtailed macaques (Macaca nemestrina): clinical, pathologic, and virologic features.. Comparative medicine. 59(5):482-7.
Travi G, Pergam SA, Xie H, Carpenter P, Kiem H-P, Corey L, Boeckh MJ.  2009.  The effect of imatinib on cytomegalovirus reactivation in hematopoietic cell transplantation.. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 49(11):e120-3.
Thakar MS, Zhang X-B, Beard BC, Sale GE, Santos EB, Peterson L, Kiem H-P, Sandmaier BM.  2009.  Transmission and expansion of HOXB4-induced leukemia in two immunosuppressed dogs: implications for a new canine leukemia model.. Experimental hematology. 37(10):1157-66.
Vigorito AC, Campregher PV, Storer BE, Carpenter PA, Moravec CK, Kiem H-P, Fero ML, Warren EH, Lee SJ, Appelbaum FR et al..  2009.  Evaluation of NIH consensus criteria for classification of late acute and chronic GVHD.. Blood. 114(3):702-8.
Carnevale-Schianca F, Leisenring W, Martin PJ, Furlong T, Schoch G, Anasetti C, Appelbaum FR, Carpenter PA, Deeg JH, Kiem H-P et al..  2009.  Longitudinal assessment of morbidity and acute graft-versus-host disease after allogeneic hematopoietic cell transplantation: retrospective analysis of a multicenter phase III study.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 15(6):749-56.
Bonig H, Watts KL, Chang K-H, Kiem H-P, Papayannopoulou T.  2009.  Concurrent blockade of alpha4-integrin and CXCR4 in hematopoietic stem/progenitor cell mobilization.. Stem cells (Dayton, Ohio). 27(4):836-7.
Li ZY, Liu Y, Tuve S, Xun Y, Fan X, Min L, Feng Q, Kiviat N, Kiem H-P, Disis ML et al..  2009.  Toward a stem cell gene therapy for breast cancer.. Blood. 113(22):5423-33.
Bonig H, Priestley GV, Wohlfahrt M, Kiem H-P, Papayannopoulou T.  2009.  Blockade of alpha6-integrin reveals diversity in homing patterns among human, baboon, and murine cells.. Stem cells and development. 18(6):839-44.
Trobridge GD, Wu RA, Beard BC, Chiu SY, Muñoz NM, von Laer D, Rossi JJ, Kiem H-P.  2009.  Protection of stem cell-derived lymphocytes in a primate AIDS gene therapy model after in vivo selection.. PloS one. 4(11):e7693.
Muñoz NM, Trobridge GD, Kiem H-P.  2009.  Ex vivo expansion and lentiviral transduction of Macaca nemestrina CD4+ T cells.. Journal of medical primatology. 38(6):438-43.
Watts KL, Beard BC, Wood BL, Kiem HP.  2009.  Myeloablative irradiation in non-human primates.. Journal of medical primatology. 38(6):425-32.

2008

Mezquita P, Beard BC, Kiem H-P.  2008.  NOD/SCID repopulating cells contribute only to short-term repopulation in the baboon.. Gene therapy. 15(21):1460-2.
Trobridge GD, Beard BC, Gooch C, Wohlfahrt M, Olsen P, Fletcher J, Malik P, Kiem H-P.  2008.  Efficient transduction of pigtailed macaque hematopoietic repopulating cells with HIV-based lentiviral vectors.. Blood. 111(12):5537-43.
Zhang X-B, Beard BC, Trobridge GD, Wood BL, Sale GE, Sud R, Humphries KR, Kiem H-P.  2008.  High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector.. The Journal of clinical investigation. 118(4):1502-10.
Piasecki JC, Beagles K, Beard BC, Riddell S, Kiem H-P.  2008.  Induction of transgene-specific cytotoxic T lymphocyte responses after transplantation of gene-modified CD34+ cells despite nonablative immunosuppressive conditioning.. Human gene therapy. 19(1):103-7.
Flowers MED, Storer B, Carpenter P, Rezvani AR, Vigorito AC, Campregher PV, Moravec C, Kiem H-P, Fero M, Georges G et al..  2008.  Treatment change as a predictor of outcome among patients with classic chronic graft-versus-host disease.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 14(12):1380-4.
Seal S, Hockenbery DM, Spaulding EY, Kiem H-P, Abbassi N, Deeg JH.  2008.  Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals.. Experimental hematology. 36(12):1660-72.
Furlong T, Kiem H-P, Appelbaum FR, Carpenter PA, Deeg JH, Doney K, Flowers MED, Mielcarek M, Nash RA, Storb R et al..  2008.  Sirolimus in combination with cyclosporine or tacrolimus plus methotrexate for prevention of graft-versus-host disease following hematopoietic cell transplantation from unrelated donors.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 14(5):531-7.
Wang H, Cao H, Wohlfahrt M, Kiem H-P, Lieber A.  2008.  Tightly regulated gene expression in human hematopoietic stem cells after transduction with helper-dependent Ad5/35 vectors.. Experimental hematology. 36(7):823-31.

2007

Kiem H-P, Allen J, Trobridge G, Olson E, Keyser K, Peterson L, Russell DW.  2007.  Foamy-virus-mediated gene transfer to canine repopulating cells.. Blood. 109(1):65-70.
Sangiolo D, Lesnikova M, Nash RA, Jensen MC, Nikitine A, Kiem H-P, Georges GE.  2007.  Lentiviral vector conferring resistance to mycophenolate mofetil and sensitivity to ganciclovir for in vivo T-cell selection.. Gene therapy. 14(21):1549-54.
Shepherd BE, Kiem H-P, Lansdorp PM, Dunbar CE, Aubert G, LaRochelle A, Seggewiss R, Guttorp P, Abkowitz JL.  2007.  Hematopoietic stem-cell behavior in nonhuman primates.. Blood. 110(6):1806-13.
Ying SX, Seal S, Abbassi N, Hockenbery DM, Kiem H-P, Li X, Pagel JM, Gopal AK, Deeg HJ.  2007.  Differential effects of bexarotene on intrinsic and extrinsic pathways in TRAIL-induced apoptosis in two myeloid leukemia cell lines.. Leukemia & lymphoma. 48(5):1003-14.
Beard BC, Dickerson D, Beebe K, Gooch C, Fletcher J, Okbinoglu T, Miller DG, Jacobs MA, Kaul R, Kiem H-P et al..  2007.  Comparison of HIV-derived lentiviral and MLV-based gammaretroviral vector integration sites in primate repopulating cells.. Molecular therapy : the journal of the American Society of Gene Therapy. 15(7):1356-65.
Aker M, Tubb J, Groth AC, Bukovsky AA, Bell AC, Felsenfeld G, Kiem H-P, Stamatoyannopoulos G, Emery DW.  2007.  Extended core sequences from the cHS4 insulator are necessary for protecting retroviral vectors from silencing position effects.. Human gene therapy. 18(4):333-43.
Horn PA, Figueiredo C, Kiem H-P.  2007.  Gene therapy in the transplantation of allogeneic organs and stem cells.. Current gene therapy. 7(6):458-68.
Wohlfahrt ME, Beard BC, Lieber A, Kiem H-P.  2007.  A capsid-modified, conditionally replicating oncolytic adenovirus vector expressing TRAIL Leads to enhanced cancer cell killing in human glioblastoma models.. Cancer research. 67(18):8783-90.
Keyser KA, Beagles KE, Kiem H-P.  2007.  Comparison of mesenchymal stem cells from different tissues to suppress T-cell activation.. Cell transplantation. 16(5):555-62.
Jung CW, Beard BC, Morris JC, Neff T, Beebe K, Storer BE, Kiem H-P.  2007.  Hematopoietic stem cell engraftment: a direct comparison between intramarrow and intravenous injection in nonhuman primates.. Experimental hematology. 35(7):1132-9.
Gerull S, Beard BC, Peterson LJ, Neff T, Kiem H-P.  2007.  In vivo selection and chemoprotection after drug resistance gene therapy in a nonmyeloablative allogeneic transplantation setting in dogs.. Human gene therapy. 18(5):451-6.
Beard BC, Keyser KA, Trobridge GD, Peterson LJ, Miller DG, Jacobs M, Kaul R, Kiem H-P.  2007.  Unique integration profiles in a canine model of long-term repopulating cells transduced with gammaretrovirus, lentivirus, or foamy virus.. Human gene therapy. 18(5):423-34.
Zhang X-B, Schwartz JL, Humphries KR, Kiem H-P.  2007.  Effects of HOXB4 overexpression on ex vivo expansion and immortalization of hematopoietic cells from different species.. Stem cells (Dayton, Ohio). 25(8):2074-81.
Neff T, Gerull S, Peterson LJ, Kiem H-P.  2007.  Improved short-term engraftment of lentivirally versus gammaretrovirally transduced allogeneic canine repopulating cells.. The journal of gene medicine. 9(5):357-61.
Bonfim CM, de Medeiros CR, Bitencourt MA, Zanis-Neto J, Funke VAM, Setubal DC, Ruiz J, Sanders JE, Flowers MED, Kiem H-P et al..  2007.  HLA-matched related donor hematopoietic cell transplantation in 43 patients with Fanconi anemia conditioned with 60 mg/kg of cyclophosphamide.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 13(12):1455-60.

2006

Horn PA, Kiem H-P.  2006.  Expansion of SCID repopulating cells does not prove expansion of hematopoietic stem cells.. Blood. 108(2):771;authorreply771-2.
Zhang X-B, Beard BC, Beebe K, Storer B, Humphries KR, Kiem H-P.  2006.  Differential effects of HOXB4 on nonhuman primate short- and long-term repopulating cells.. PLoS medicine. 3(5):e173.
Beard BC, Mezquita P, Morris JC, Kiem H-P.  2006.  Efficient transduction and engraftment of G-CSF-mobilized peripheral blood CD34+ cells in nonhuman primates using GALV-pseudotyped gammaretroviral vectors.. Molecular therapy : the journal of the American Society of Gene Therapy. 14(2):212-7.
Neff T, Beard BC, Kiem H-P.  2006.  Survival of the fittest: in vivo selection and stem cell gene therapy.. Blood. 107(5):1751-60.
Placantonakis DG, Bukovsky AA, Aicher SA, Kiem H-P, Welsh JP.  2006.  Continuous electrical oscillations emerge from a coupled network: a study of the inferior olive using lentiviral knockdown of connexin36.. The Journal of neuroscience : the official journal of the Society for Neuroscience. 26(19):5008-16.
Kurre P, Anandakumar P, Kiem H-P.  2006.  Rapid 1-hour transduction of whole bone marrow leads to long-term repopulation of murine recipients with lentivirus-modified hematopoietic stem cells.. Gene therapy. 13(4):369-73.
Spence AM, Kiem H-P, Partap S, Schuetze S, Silber JR, Peterson RA.  2006.  Complications of a temozolomide overdose: a case report.. Journal of neuro-oncology. 80(1):57-61.
Deeg JH, Storer BE, Boeckh M, Martin PJ, McCune JS, Myerson D, Heimfeld S, Flowers ME, Anasetti C, Doney KC et al..  2006.  Reduced incidence of acute and chronic graft-versus-host disease with the addition of thymoglobulin to a targeted busulfan/cyclophosphamide regimen.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 12(5):573-84.
Trobridge GD, Miller DG, Jacobs MA, Allen JM, Kiem H-P, Kaul R, Russell DW.  2006.  Foamy virus vector integration sites in normal human cells.. Proceedings of the National Academy of Sciences of the United States of America. 103(5):1498-503.
Storek J, Kiem H-P.  2006.  Transgene expression by a large fraction of dendritic cells following autologous transplantation of retrovirally transduced CD34 cells.. Stem cells and development. 15(5):619-21.

2005

Trobridge G, Beard BC, Kiem H-P.  2005.  Hematopoietic stem cell transduction and amplification in large animal models.. Human gene therapy. 16(12):1355-66.
Keyser KA, Morris JC, Kiem H-P.  2005.  Genetically modified CD34+ cells do not contribute to the mesenchymal compartment after autologous transplantation in the baboon.. Cytotherapy. 7(4):345-52.
Beagles KE, Peterson L, Zhang X, Morris J, Kiem H-P.  2005.  Cyclosporine inhibits the development of green fluorescent protein (GFP)-specific immune responses after transplantation of GFP-expressing hematopoietic repopulating cells in dogs.. Human gene therapy. 16(6):725-33.
Harkey MA, Czerwinski M, Slattery J, Kiem H-P.  2005.  Overexpression of glutathione-S-transferase, MGSTII, confers resistance to busulfan and melphalan.. Cancer investigation. 23(1):19-25.
Nash RA, Johnston L, Parker P, McCune JS, Storer B, Slattery JT, Furlong T, Anasetti C, Appelbaum FR, Lloid ME et al..  2005.  A phase I/II study of mycophenolate mofetil in combination with cyclosporine for prophylaxis of acute graft-versus-host disease after myeloablative conditioning and allogeneic hematopoietic cell transplantation.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 11(7):495-505.
Neff T, Beard BC, Peterson LJ, Anandakumar P, Thompson J, Kiem H-P.  2005.  Polyclonal chemoprotection against temozolomide in a large-animal model of drug resistance gene therapy.. Blood. 105(3):997-1002.
Zanis-Neto J, Flowers MED, Medeiros CR, Bitencourt MA, Bonfim CM, Setúbal DC, Funke V, Sanders J, Deeg HJ, Kiem HP et al..  2005.  Low-dose cyclophosphamide conditioning for haematopoietic cell transplantation from HLA-matched related donors in patients with Fanconi anaemia.. British journal of haematology. 130(1):99-106.
Ni S, Bernt K, Gaggar A, Li Z-Y, Kiem H-P, Lieber A.  2005.  Evaluation of biodistribution and safety of adenovirus vectors containing group B fibers after intravenous injection into baboons.. Human gene therapy. 16(6):664-77.
Lu H, Zhao Z, Kalina T, Gillespy T, Liggitt D, Andrews RG, Maloney DG, Kiem H-P, Storek J.  2005.  Interleukin-7 improves reconstitution of antiviral CD4 T cells.. Clinical immunology (Orlando, Fla.). 114(1):30-41.
Kalina T, Lu H, Zhao Z, Blewett E, Dittmer DP, Randolph-Habecker J, Maloney DG, Andrews RG, Kiem H-P, Storek J.  2005.  De novo generation of CD4 T cells against viruses present in the host during immune reconstitution.. Blood. 105(6):2410-4.

2004

Horn PA, Keyser KA, Peterson LJ, Neff T, Thomasson BM, Thompson J, Kiem H-P.  2004.  Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol.. Blood. 103(10):3710-6.
Platzbecker U, Kurre P, Guardiola P, Ward JL, Radich JP, Kiem H-P, Deeg JH.  2004.  Fanconi anemia type C-deficient hematopoietic cells are resistant to TRAIL (TNF-related apoptosis-inducing ligand)-induced cleavage of pro-caspase-8.. Experimental hematology. 32(9):815-21.
Stewart BL, Storer B, Storek J, Deeg JH, Storb R, Hansen JA, Appelbaum FR, Carpenter PA, Sanders JE, Kiem H-P et al..  2004.  Duration of immunosuppressive treatment for chronic graft-versus-host disease.. Blood. 104(12):3501-6.
Schielke JE, Kiem H-P, Liggitt D, Bielefeldt-Ohmann H.  2004.  Radiation-induced glioblastoma multiforme in two adult baboons (Papio cynocephalus anubis).. Comparative medicine. 54(3):327-32.
Morris JC, Conerly M, Thomasson B, Storek J, Riddell SR, Kiem H-P.  2004.  Induction of cytotoxic T-lymphocyte responses to enhanced green and yellow fluorescent proteins after myeloablative conditioning.. Blood. 103(2):492-9.
Placantonakis DG, Bukovsky AA, Zeng X-H, Kiem H-P, Welsh JP.  2004.  Fundamental role of inferior olive connexin 36 in muscle coherence during tremor.. Proceedings of the National Academy of Sciences of the United States of America. 101(18):7164-9.
Horn PA, Morris JC, Neff T, Kiem H-P.  2004.  Stem cell gene transfer--efficacy and safety in large animal studies.. Molecular therapy : the journal of the American Society of Gene Therapy. 10(3):417-31.
Kurre P, Anandakumar P, Harkey MA, Thomasson B, Kiem H-P.  2004.  Efficient marking of murine long-term repopulating stem cells targeting unseparated marrow cells at low lentiviral vector particle concentration.. Molecular therapy : the journal of the American Society of Gene Therapy. 9(6):914-22.
Bielefeldt-Ohmann H, Gough M, Durning M, Kelley S, Liggitt HD, Kiem H-P.  2004.  Greater sensitivity of pigtailed macaques (Macaca nemestrina) than baboons to total body irradiation.. Journal of comparative pathology. 131(1):77-86.
Kiem H-P, Sellers S, Thomasson B, Morris JC, Tisdale JF, Horn PA, Hematti P, Adler R, Kuramoto K, Calmels B et al..  2004.  Long-term clinical and molecular follow-up of large animals receiving retrovirally transduced stem and progenitor cells: no progression to clonal hematopoiesis or leukemia.. Molecular therapy : the journal of the American Society of Gene Therapy. 9(3):389-95.
Richard RE, De Claro AR, Yan J, Chien S, Von Recum H, Morris J, Kiem H-P, Dalgarno DC, Heimfeld S, Clackson T et al..  2004.  Differences in F36VMpl-based in vivo selection among large animal models.. Molecular therapy : the journal of the American Society of Gene Therapy. 10(4):730-40.
Neff T, Peterson LJ, Morris JC, Thompson J, Zhang X, Horn PA, Thomasson BM, Kiem H-P.  2004.  Efficient gene transfer to hematopoietic repopulating cells using concentrated RD114-pseudotype vectors produced by human packaging cells.. Molecular therapy : the journal of the American Society of Gene Therapy. 9(2):157-9.
Walter RB, Raden BW, Thompson J, Flowers DA, Kiem H-P, Bernstein ID, Linenberger ML.  2004.  Breast cancer resistance protein (BCRP/ABCG2) does not confer resistance to gemtuzumab ozogamicin and calicheamicin-gamma1 in acute myeloid leukemia cells.. Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K. 18(11):1914-7.

2003

Thomasson B, Peterson L, Thompson J, Goerner M, Kiem H-P.  2003.  Direct comparison of steady-state marrow, primed marrow, and mobilized peripheral blood for transduction of hematopoietic stem cells in dogs.. Human gene therapy. 14(17):1683-6.
Neff T, Horn PA, Peterson LJ, Thomasson BM, Thompson J, Williams DA, Schmidt M, Georges GE, von Kalle C, Kiem H-P.  2003.  Methylguanine methyltransferase-mediated in vivo selection and chemoprotection of allogeneic stem cells in a large-animal model.. The Journal of clinical investigation. 112(10):1581-8.
Kurre P, Morris J, Thomasson B, Kohn DB, Kiem H-P.  2003.  Scaffold attachment region-containing retrovirus vectors improve long-term proviral expression after transplantation of GFP-modified CD34+ baboon repopulating cells.. Blood. 102(9):3117-9.
Tiede A, Eder M, von Depka M, Battmer K, Luther S, Kiem H-P, Ganser A, Scherr M.  2003.  Recombinant factor VIII expression in hematopoietic cells following lentiviral transduction.. Gene therapy. 10(22):1917-25.
Horn PA, Thomasson BM, Wood BL, Andrews RG, Morris JC, Kiem H-P.  2003.  Distinct hematopoietic stem/progenitor cell populations are responsible for repopulating NOD/SCID mice compared with nonhuman primates.. Blood. 102(13):4329-35.
Deeg JH, Gooley TA, Flowers MED, Sale GE, Slattery JT, Anasetti C, Chauncey TR, Doney K, Georges GE, Kiem H-P et al..  2003.  Allogeneic hematopoietic stem cell transplantation for myelofibrosis.. Blood. 102(12):3912-8.
Storek J, Gillespy T, Lu H, Joseph A, Dawson MA, Gough M, Morris J, Hackman RC, Horn PA, Sale GE et al..  2003.  Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys.. Blood. 101(10):4209-18.
Kohn DB, Sadelain M, Dunbar C, Bodine D, Kiem H-P, Candotti F, Tisdale J, Riviére I, Blau AC, Richard RE et al..  2003.  American Society of Gene Therapy (ASGT) ad hoc subcommittee on retroviral-mediated gene transfer to hematopoietic stem cells.. Molecular therapy : the journal of the American Society of Gene Therapy. 8(2):180-7.
Kurre P, Pulsipher M, Woolfrey A, Maris M, Sandmaier B, Kiem H-P, Storb R.  2003.  Reduced toxicity and prompt engraftment after minimal conditioning of a patient with Fanconi anemia undergoing hematopoietic stem cell transplantation from an HLA-matched unrelated donor.. Journal of pediatric hematology/oncology : official journal of the American Society of Pediatric Hematology/Oncology. 25(7):581-3.
Schmidt M, Glimm H, Wissler M, Hoffmann G, Olsson K, Sellers S, Carbonaro D, Tisdale JF, Leurs C, Hanenberg H et al..  2003.  Efficient characterization of retro-, lenti-, and foamyvector-transduced cell populations by high-accuracy insertion site sequencing.. Annals of the New York Academy of Sciences. 996:112-21.

2002

Kurre P, Anandakumar P, Grompe M, Kiem HP.  2002.  In vivo administration of interferon gamma does not cause marrow aplasia in mice with a targeted disruption of FANCC.. Experimental hematology. 30(11):1257-62.
Horn PA, Topp MS, Morris JC, Riddell SR, Kiem H-P.  2002.  Highly efficient gene transfer into baboon marrow repopulating cells using GALV-pseudotype oncoretroviral vectors produced by human packaging cells.. Blood. 100(12):3960-7.
Horn PA, Morris JC, Bukovsky AA, Andrews RG, Naldini L, Kurre P, Kiem H-P.  2002.  Lentivirus-mediated gene transfer into hematopoietic repopulating cells in baboons.. Gene therapy. 9(21):1464-71.
Schmidt M, Zickler P, Hoffmann G, Haas S, Wissler M, Muessig A, Tisdale JF, Kuramoto K, Andrews RG, Wu T et al..  2002.  Polyclonal long-term repopulating stem cell clones in a primate model.. Blood. 100(8):2737-43.
Kurre P, Morris J, Andrews RG, Kohn DB, Kiem H-P.  2002.  Kinetics of fluorescence expression in nonhuman primates transplanted with GFP retrovirus-modified CD34 cells.. Molecular therapy : the journal of the American Society of Gene Therapy. 6(1):83-90.
Neff T, Horn PA, Valli VE, Gown AM, Wardwell S, Wood BL, von Kalle C, Schmidt M, Peterson LJ, Morris JC et al..  2002.  Pharmacologically regulated in vivo selection in a large animal.. Blood. 100(6):2026-31.
Kiem H-P, Rasko JEJ, Morris J, Peterson L, Kurre P, Andrews RG.  2002.  Ex vivo selection for oncoretrovirally transduced green fluorescent protein-expressing CD34-enriched cells increases short-term engraftment of transduced cells in baboons.. Human gene therapy. 13(8):891-9.
Kondo E, Topp MS, Kiem H-P, Obata Y, Morishima Y, Kuzushima K, Tanimoto M, Harada M, Takahashi T, Akatsuka Y.  2002.  Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells.. Journal of immunology (Baltimore, Md. : 1950). 169(4):2164-71.
Deeg JH, Storer B, Slattery JT, Anasetti C, Doney KC, Hansen JA, Kiem H-P, Martin PJ, Petersdorf E, Radich JP et al..  2002.  Conditioning with targeted busulfan and cyclophosphamide for hemopoietic stem cell transplantation from related and unrelated donors in patients with myelodysplastic syndrome.. Blood. 100(4):1201-7.
Furlong T, Leisenring W, Storb R, Anasetti C, Appelbaum FR, Carpenter PA, Deeg JH, Doney K, Kiem H-P, Nash RA et al..  2002.  Psoralen and ultraviolet A irradiation (PUVA) as therapy for steroid-resistant cutaneous acute graft-versus-host disease.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 8(4):206-12.
Goerner M, Gooley T, Flowers MED, Sullivan KM, Kiem H-P, Sanders JE, Martin PJ, Storb R.  2002.  Morbidity and mortality of chronic GVHD after hematopoietic stem cell transplantation from HLA-identical siblings for patients with aplastic or refractory anemias.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 8(1):47-56.

2001

Georges GE, Storb R, Bruno B, Brodie SJ, Thompson JD, Taranova AG, Zaucha JM, Little MT, Zellmer E, Moore PF et al..  2001.  Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes.. Blood. 98(12):3447-55.
Berger C, Huang ML, Gough M, Greenberg PD, Riddell SR, Kiem HP.  2001.  Nonmyeloablative immunosuppressive regimen prolongs In vivo persistence of gene-modified autologous T cells in a nonhuman primate model.. Journal of virology. 75(2):799-808.
Feinstein L, Sandmaier B, Maloney D, McSweeney PA, Maris M, Flowers C, Radich J, Little MT, Nash RA, Chauncey T et al..  2001.  Nonmyeloablative hematopoietic cell transplantation. Replacing high-dose cytotoxic therapy by the graft-versus-tumor effect.. Annals of the New York Academy of Sciences. 938:328-37;discussion337-9.
McSweeney PA, Niederwieser D, Shizuru JA, Sandmaier BM, Molina AJ, Maloney DG, Chauncey TR, Gooley TA, Hegenbart U, Nash RA et al..  2001.  Hematopoietic cell transplantation in older patients with hematologic malignancies: replacing high-dose cytotoxic therapy with graft-versus-tumor effects.. Blood. 97(11):3390-400.
Zaucha JA, Yu C, Lothrop CD, Nash RA, Sale G, Georges G, Kiem HP, Niemeyer GP, Dufresne M, Cao Q et al..  2001.  Severe canine hereditary hemolytic anemia treated by nonmyeloablative marrow transplantation.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 7(1):14-24.
Purton LE, Morris JC, Bernstein ID, Collins SJ, Kiem HP.  2001.  All-trans retinoic acid facilitates oncoretrovirus-mediated transduction of hematopoietic repopulating stem cells.. Journal of hematotherapy & stem cell research. 10(6):815-25.
Goerner M, Horn PA, Peterson L, Kurre P, Storb R, Rasko JE, Kiem HP.  2001.  Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors.. Blood. 98(7):2065-70.
Kurre P, Morris J, Horn PA, Harkey MA, Andrews RG, Kiem HP.  2001.  Gene transfer into baboon repopulating cells: A comparison of Flt-3 Ligand and megakaryocyte growth and development factor versus IL-3 during ex vivo transduction.. Molecular therapy : the journal of the American Society of Gene Therapy. 3(6):920-7.
Kurre P, Morris J, Miller AD, Kiem HP.  2001.  Envelope fusion protein binding studies in an inducible model of retrovirus receptor expression and in CD34(+) cells emphasize limited transduction at low receptor levels.. Gene therapy. 8(8):593-9.
Bruno B, Goerner MA, Nash RA, Storb R, Kiem HP, McSweeney PA.  2001.  Purified canine CD34+Lin- marrow cells transduced with retroviral vectors give rise to long-term multi-lineage hematopoiesis.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 7(10):543-51.

2000

Kurre P, Kiem HP.  2000.  Progress towards hematopoietic stem cell gene therapy.. Current opinion in molecular therapeutics. 2(4):400-11.
Goerner M, Roecklein B, Torok-Storb B, Heimfeld S, Kiem HP.  2000.  Expansion and transduction of nonenriched human cord blood cells using HS-5 conditioned medium and FLT3-L.. Journal of hematotherapy & stem cell research. 9(5):759-65.
Andrews RG, Peterson LJ, Morris J, Potter J, Heyward S, Gough M, Bryant E, Kiem HP.  2000.  Differential engraftment of genetically modified CD34(+) and CD34(-) hematopoietic cell subsets in lethally irradiated baboons.. Experimental hematology. 28(5):508-18.
Shields LE, Kiem HP, Andrews RG.  2000.  Highly efficient gene transfer into preterm CD34 hematopoietic progenitor cells.. American journal of obstetrics and gynecology. 183(3):732-7.
Berger C, Xuereb S, Johnson DC, Watanabe KS, Kiem HP, Greenberg PD, Riddell SR.  2000.  Expression of herpes simplex virus ICP47 and human cytomegalovirus US11 prevents recognition of transgene products by CD8(+) cytotoxic T lymphocytes.. Journal of virology. 74(10):4465-73.

1999

Goerner M, Bruno B, McSweeney PA, Buron G, Storb R, Kiem HP.  1999.  The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene transfer into hematopoietic repopulating cells in dogs.. Blood. 94(7):2287-92.
Kiem HP, McSweeney PA, Bruno B, Goerner M, Buron G, Morris J, Storb R, Miller AD.  1999.  Improved gene transfer into canine hematopoietic repopulating cells using CD34-enriched marrow cells in combination with a gibbon ape leukemia virus-pseudotype retroviral vector.. Gene therapy. 6(6):966-72.
Storb R, Yu C, Zaucha JM, Deeg HJ, Georges G, Kiem HP, Nash RA, McSweeney PA, Wagner JL.  1999.  Stable mixed hematopoietic chimerism in dogs given donor antigen, CTLA4Ig, and 100 cGy total body irradiation before and pharmacologic immunosuppression after marrow transplant.. Blood. 94(7):2523-9.
Storb R, Yu C, Barnett T, Wagner JL, Deeg HJ, Nash RA, Kiem HP, McSweeney P, Seidel K, Georges G et al..  1999.  Stable mixed hematopoietic chimerism in dog leukocyte antigen-identical littermate dogs given lymph node irradiation before and pharmacologic immunosuppression after marrow transplantation.. Blood. 94(3):1131-6.
Winkler A, Kiem HP, Shields LE, Sun QH, Andrews RG.  1999.  Gene transfer into fetal baboon hematopoietic progenitor cells.. Human gene therapy. 10(4):667-77.
Kurre P, Kiem HP, Morris J, Heyward S, Battini JL, Miller AD.  1999.  Efficient transduction by an amphotropic retrovirus vector is dependent on high-level expression of the cell surface virus receptor.. Journal of virology. 73(1):495-500.

1998

Kiem HP, Andrews RG, Morris J, Peterson L, Heyward S, Allen JM, Rasko JE, Potter J, Miller AD.  1998.  Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor.. Blood. 92(6):1878-86.
Georges GE, Storb R, Brunvand MW, Kiem HP, Moore PF, Malik P, Ennist D, Nash RA.  1998.  Canine T cells transduced with a herpes simplex virus thymidine kinase gene: a model to study effects on engraftment and control of graft-versus-host disease.. Transplantation. 66(4):540-4.
Storb R, Yu C, Deeg HJ, Georges G, Kiem HP, McSweeney PA, Nash RA, Sandmaier BM, Sullivan KM, Wagner JL et al..  1998.  Current and future preparative regimens for bone marrow transplantation in thalassemia.. Annals of the New York Academy of Sciences. 850:276-87.
Glimm H, Flügge K, Möbest D, Hofmann VM, Postmus J, Henschler R, Lange W, Finke J, Kiem HP, Schulz G et al..  1998.  Efficient serum-free retroviral gene transfer into primitive human hematopoietic progenitor cells by a defined, high-titer, nonconcentrated vector-containing medium.. Human gene therapy. 9(6):771-8.

1997

Dybing J, Lynch CM, Hara P, Jurus L, Kiem HP, Anklesaria P.  1997.  GaLV pseudotyped vectors and cationic lipids transduce human CD34+ cells.. Human gene therapy. 8(14):1685-94.
Storb R, Yu C, Wagner JL, Deeg HJ, Nash RA, Kiem HP, Leisenring W, Shulman H.  1997.  Stable mixed hematopoietic chimerism in DLA-identical littermate dogs given sublethal total body irradiation before and pharmacological immunosuppression after marrow transplantation.. Blood. 89(8):3048-54.
Czerwinski M, Kiem HP, Slattery JT.  1997.  Human CD34+ cells do not express glutathione S-transferases alpha.. Gene therapy. 4(3):268-70.
Kiem HP, Heyward S, Winkler A, Potter J, Allen JM, Miller AD, Andrews RG.  1997.  Gene transfer into marrow repopulating cells: comparison between amphotropic and gibbon ape leukemia virus pseudotyped retroviral vectors in a competitive repopulation assay in baboons.. Blood. 90(11):4638-45.
Kiem HP, Storb R, McDonald GB.  1997.  Hepatitis-associated aplastic anemia.. The New England journal of medicine. 337(6):424-5.
Kiem HP, Myerson D, Storb R, McDonald GB, Spurgeon CL, Leisenring W.  1997.  Prevalence of hepatitis G virus in patients with aplastic anemia.. Blood. 90(3):1335-6.
Schuening F, Longo WL, Atkinson ME, Zaboikin M, Kiem HP, Sanders J, Scott CR, Storb R, Miller AD, Reynolds T et al..  1997.  Retrovirus-mediated transfer of the cDNA for human glucocerebrosidase into peripheral blood repopulating cells of patients with Gaucher's disease.. Human gene therapy. 8(17):2143-60.
Glimm H, Kiem HP, Darovsky B, Storb R, Wolf J, Diehl V, Mertelsmann R, Von Kalle C.  1997.  Efficient gene transfer in primitive CD34+/CD38lo human bone marrow cells reselected after long-term exposure to GALV-pseudotyped retroviral vector.. Human gene therapy. 8(17):2079-86.
Schuening FG, Von Kalle C, Kiem HP, Appelbaum FR, Deeg HJ, Pepe M, Gooley T, Graham TC, Hackman RC, Storb R.  1997.  Effect of recombinant canine stem cell factor, a c-kit ligand, on hematopoietic recovery after DLA-identical littermate marrow transplants in dogs.. Experimental hematology. 25(12):1240-5.

1996

Shull R, Lu X, Dubé I, Lutzko C, Kruth S, Abrams-Ogg A, Kiem HP, Goehle S, Schuening F, Millan C et al..  1996.  Humoral immune response limits gene therapy in canine MPS I.. Blood. 88(1):377-9.
Miller AD, Bonham L, Alfano J, Kiem HP, Reynolds T, Wolgamot G.  1996.  A novel murine retrovirus identified during testing for helper virus in human gene transfer trials.. Journal of virology. 70(3):1804-9.
Kiem HP, Leisenring W, Raff R, Deeg HJ, Schuening FG, Appelbaum FR, Storb R.  1996.  Failure of recombinant stem cell factor to enhance engraftment of L-leucyl-L-leucine methyl ester treated canine marrow after irradiation.. Blood. 88(5):1896-7.
Kiem HP, McDonald GB, Myerson D, Spurgeon CL, Deeg HJ, Sanders JE, Doney K, Appelbaum FR, Sullivan KM, Witherspoon RP et al..  1996.  Marrow transplantation for hepatitis-associated aplastic anemia: a follow-up of long-term survivors.. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 2(2):93-9.
Kiem HP, Darovsky B, Von Kalle C, Goehle S, Graham T, Miller AD, Storb R, Schuening FG.  1996.  Long-term persistence of canine hematopoietic cells genetically marked by retrovirus vectors.. Human gene therapy. 7(1):89-96.

1995

Wagner LA, Kiem HP, McSweeney P, Schuening FG, Storb R, Ostrander EA.  1995.  Development of a clonality assay for canine hematopoietic stem cells.. Blood. 86(8):3265-7.
Barquinero J, Kiem HP, Von Kalle C, Darovsky B, Goehle S, Graham T, Seidel K, Storb R, Schuening FG.  1995.  Myelosuppressive conditioning improves autologous engraftment of genetically marked hematopoietic repopulating cells in dogs.. Blood. 85(5):1195-201.
Kiem HP, Von Kalle C, Schuening F, Storb R.  1995.  Gene therapy and bone marrow transplantation.. Current opinion in oncology. 7(2):107-14.

1994

Von Kalle C, Kiem HP, Goehle S, Darovsky B, Heimfeld S, Torok-Storb B, Storb R, Schuening FG.  1994.  Increased gene transfer into human hematopoietic progenitor cells by extended in vitro exposure to a pseudotyped retroviral vector.. Blood. 84(9):2890-7.
Kiem HP, Darovsky B, Von Kalle C, Goehle S, Stewart D, Graham T, Hackman R, Appelbaum FR, Deeg HJ, Miller AD.  1994.  Retrovirus-mediated gene transduction into canine peripheral blood repopulating cells.. Blood. 83(6):1467-73.

1991

Schmidt-Wolf IG, Negrin RS, Kiem HP, Blume KG, Weissman IL.  1991.  Use of a SCID mouse/human lymphoma model to evaluate cytokine-induced killer cells with potent antitumor cell activity.. The Journal of experimental medicine. 174(1):139-49.
Negrin RS, Kiem HP, Schmidt-Wolf IG, Blume KG, Cleary ML.  1991.  Use of the polymerase chain reaction to monitor the effectiveness of ex vivo tumor cell purging.. Blood. 77(3):654-60.

1990

Kiem HP, Nourse J, Saltman DL, Blume KG, Cleary ML.  1990.  Concurrent activation of c-myc and inactivation of bcl-2 by chromosomal translocation in a lymphoblastic lymphoma cell line.. Oncogene. 5(12):1815-9.



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