Nucleotide sequence preservation of human mitochondrial DNA.
|Title||Nucleotide sequence preservation of human mitochondrial DNA.|
|Publication Type||Journal Article|
|Year of Publication||1985|
|Authors||Monnat RJ, Loeb LA|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Date Published||1985 May|
|Keywords||Adult, Base Sequence, Biological Evolution, Cloning, Molecular, DNA, Mitochondrial, Electron Transport Complex IV, Female, Genetic Variation, Humans, Male, Middle Aged, Zygote|
Recombinant DNA techniques have been used to quantitate the amount of nucleotide sequence divergence in the mitochondrial DNA population of individual normal humans. Mitochondrial DNA was isolated from the peripheral blood lymphocytes of five normal humans and cloned in M13 mp11; 49 kilobases of nucleotide sequence information was obtained from 248 independently isolated clones from the five normal donors. Both between- and within-individual differences were identified. Between-individual differences were identified in approximately 1/200 nucleotides. In contrast, only one within-individual difference was identified in 49 kilobases of nucleotide sequence information. This high degree of mitochondrial nucleotide sequence homogeneity in human somatic cells is in marked contrast to the rapid evolutionary divergence of human mitochondrial DNA and suggests the existence of mechanisms for the concerted preservation of mammalian mitochondrial DNA sequences in single organisms.
|Alternate Journal||Proc. Natl. Acad. Sci. U.S.A.|