GaLV pseudotyped vectors and cationic lipids transduce human CD34+ cells.
|Title||GaLV pseudotyped vectors and cationic lipids transduce human CD34+ cells.|
|Publication Type||Journal Article|
|Year of Publication||1997|
|Authors||Dybing J, Lynch CM, Hara P, Jurus L, Kiem HP, Anklesaria P|
|Journal||Human gene therapy|
|Date Published||1997 Sep 20|
|Keywords||Alkaline Phosphatase, Antigens, CD34, Bone Marrow Cells, Cations, Cell Line, Cells, Cultured, Flow Cytometry, Gene Transfer Techniques, Genes, Reporter, Genetic Vectors, Hematopoietic Stem Cells, Hexadimethrine, Humans, Leukemia Virus, Gibbon Ape, Lipids, Moloney murine leukemia virus, Spermine|
High transduction frequency of hematopoietic stem/progenitor cells is essential to derive clinical benefits for treating certain inherited and acquired diseases. We demonstrate here stable gene transfer into human bone marrow-derived CD34+ progenitors using cationic lipids to facilitate GaLV and amphotroic pseudotyped retroviral-mediated transductions. Furthermore, the transgene was detected only in the progeny of flow cytometer sorted CD34+ population transduced by the LAPSN (PG13) viral vector in the presence of cationic lipids but not when transduction was facilitated with conventional polycations Polybrene or protamine sulfate. Thus, a combination of GaLV pseudotyped vectors and cationic lipids results in increased transduction frequencies of the CD34+ cells without a requirement of extended in vitro culture, or co-cultivation with producer cell lines. These improvements may result in the production of therapeutically significant quantities of genetically modified hematopoietic cells.
|Alternate Journal||Hum. Gene Ther.|