Acutely transforming retrovirus expressing Nras generated from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV.
|Title||Acutely transforming retrovirus expressing Nras generated from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Metzger MJ, Miller DA|
|Journal||Journal of virology|
|Date Published||2010 Aug|
|Keywords||Animals, Cell Line, Tumor, Cell Transformation, Viral, Endogenous Retroviruses, Genes, ras, Genes, Viral, Genome, Viral, Humans, Rats|
Virus from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) can induce rare foci of transformation in rat 208F fibroblasts. Characterization of three such foci revealed that one produced an acutely transforming virus at a high titer. The virus consists of a mutant Nras cDNA from the HT-1080 cells inserted into a retroviral vector (added to the HT-1080 cells as a marker for infection) in place of internal vector sequences. These results show that XMRV can generate acutely transforming viruses at a low rate, as is typical of other replication-competent retroviruses, and reveal the potential for transforming virus contamination of retroviral vectors made from transformed cell lines.
|Alternate Journal||J. Virol.|